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WebbThe present invention provides for reaction mixtures for reverse transcription of RNA in a sample. In some embodiments, the mixture comprises: a reverse transcriptase having an optimal pH above 8; and a buffer, wherein the mixture has a pH of between 6-8, lacks template RNA, and the reverse transcriptase maintains essentially all (e.g., at least 95% … WebbWHAT IS THE HIGHEST TEMPERATURE AT WHICH THERMOSCRIPT REVERSE TRANSCRIPTASE CAN BE USED?It can be used at temperatures as high as 70oC (especially for amplicons expectedto be 1 kb or less). For PCR products expected to be greater than 1 kb a maximumfirst strand synthesis temperature of 65oC is suggested.IN … drawing of ocean wave
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