WebSep 16, 2024 · The primers used for qRT-PCR are shown in Online Supplementary Table S1. The qRT-PCR cycle parameters were as follows: initial denaturation at 95°C for 10 minutes, then denaturation at 95°C for 20 seconds, annealing at 60°C for 30 seconds, extension at 72°C for 30 seconds, and finally 72°C for 10 minutes.
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