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Tīmeklis2024. gada 3. apr. · LAMP was developed in 2000 and is characterized by high specificity, sensitivity, speed (<60 min), low reaction temperature (60°–65°C), and high robustness ( 4 ). The amplification efficiency is very high, allowing the development of simple assays without requiring dedicated devices ( 5, 6 ). Tīmeklis2024. gada 1. jūn. · Herein, a visual-mixed-dye (VMD) containing calcein (precombined with MnCl 2) and hydroxynaphthol blue (HNB) for LAMP end-point detection was developed. The optimal final concentrations of these components in VMD were 25 and 300 μM, respectively. Due to the formation of HNBMn 2+ during the LAMP reaction, … lsf daemon lim not responding
A novel visual-mixed-dye for LAMP and its application in the
TīmeklisThe WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results. Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye … TīmeklisA patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Detection method/readout. PCR/qPCR, HRM®. Excitation/Emission. … Tīmeklis2024. gada 8. febr. · The Lava the Original 14.5-Inch Tie Dye Pink Spiral Lamp brings the drama of motion and the beauty of soft colored light into your very own space. … lsf dermal fillers discount code