How to make kanamycin stock solution
WebHello Everyone,I’ve created these videos primarily as instructional aides for new students, interns, and trainees in my research group. While many of the me... WebPrepare stock solutions directly in the vial with sterile water (10 mg/ml). Kanamycin sulfate is soluble in water (50 mg/ml), yielding a clear solution. Kanamycin sulfate is …
How to make kanamycin stock solution
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WebKanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II ( APH3) gene … Web4. Remove the magnetic stir bar and pour the solution into a clean 1000 ml graduated cylinder. Use dH 2 O to bring the liquid level to 1000 ml. 5. Pour the medium back to the flask and add the stir bar. 6. Add 15g of granulated agar to the liquid and stir for about a minute. 7. Remove the stir bar.
Web27 okt. 2008 · Weigh 800mg of kanamycin into a small weigh boat. Add 80ml of milliQ to a 250ml bottle. Add the kanamycin to the milliQ; Mix/vortex so all the kanamycin goes … http://breastcancerlab.com/protocols/
WebChloramphenicol - 10 mL (1,000 X Stock) 34 mg/ml chloramphenicol in ethanol. Reagents needed: 0.34 g. chloramphenicol. 10 ml. 100% ethanol. Directions: 1) Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol. 2) Filter through a 0.22 µm filter to sterilize. 3) Aliquot and store at -20°C. Web14 sep. 2016 · Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open one plate at a time next to the flame and begin pouring. Measure …
Web3 jan. 2024 · This means your plates containing methicillin, erythromycin, cephalothin, tetracycline, chloramphenicol, kanamycin, streptomycin, polymyxin B, or nalidixic acid should be a-ok even if stored for one month at 4 o C. ... Your antibiotic activity will also be affected by how you store the stock solution prior to making your agar plates.
WebLysogeny broth (LB) medium with appropriate antibiotics. LB agar medium with appropriate antibiotics • Terrific broth (TB) medium with appropriate antibiotics • Isopropyl β-d-1-thiogalactopyranoside (IPTG, 1 M in H 2 O, filter sterilized)1000 × antibiotic solutions in H 2 O (filter sterilized). Kanamycin (25 mg/mL). Spectinomycin (25 mg/mL). Ampicillin (100 … in stillness and quietness scriptureWebYou need to make 500mL of 7H9(+) media with 1X ADN and 30ug/ml. of the antibiotic kanamycin. Your stock solutions are 10X ADN and 30mg/mL of kanamycin. The solvent is distilled water. How much of cach component will you … instill in my mindWebThis protocol describes the preparation of an Ampicillin stock solution. The concentration of this stock solution is based on the weight of anhydrous Ampicillin.. Note: Typical stock solutions for this antibiotic in selectable media are made at 50mg ml-1 and are used at a final concentration of 20-40 μg ml-1 for stringent plasmids and 100 μg ml-1 for relased … instill meaning synonymWeb11 dec. 2024 · Dissolve completely. 12. 41 g/mol) Chloramphenicol Stock Solution 50mg/Ml - 99,9% pure EtOH - dH 2 O Method 1. Protect from light From the calculation, you need to pipette 4 mL of the 5 M sulfuric acid solution to prepare 10 mL of 2 M sulfuric acid solution. 13 g/mol. 3) Aliquot and store at -20°C. Chlornitromycin. jmf realty llcWebRecipe 1000X Kanamycin stock Kanamycin Make kanamycin to a final concentration of 50 mg/ml in H 2 O. Filter-sterilize and store in aliquots at −20ºC. CiteULike Delicious … jmf realtyjmf property servicesWebIX. STOCK SOLUTIONS AND DILUTIONS. Consider your 10% w/v sucrose and 1M NaCl, prepared as part of module VIII and stored in the refrigerator, as stock solutions from which other solutions can be made. EXAMPLE: MAKING 1% w/v SUCROSE FROM 10% w/v STOCK: A 1% sucrose solution can be prepared from the 10% stock by 1:10 … in stillness conquer fear